Polyclonal Antibody Development
• Synthesis of up to 15aa peptide from customer supplied sequence
• Includes conjugation of peptide to KLH via N-Terminal cysteine
• 11 week, 2 rabbit protocol
• ELISA test to determine specificity and titer
• Protein G purification

Monoclonal Antibody Development
• Synthesis of up to 15aa peptide from customer supplied sequence
• Includes conjugation of peptide to KLH via N-Terminal cysteine
• Hybridoma fusion, screening, subcloning and expansion
• Purification by Protein G

*Please inquire for optional services, including affinity purification, extended immunization schedule, and synthesis of additional peptide.

Bacteria Protein Expression
• Clone gene of interest into an expression vector
• Generation and identification of bacterial colonies expressing target proteins
• Large-scale culture
• Purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione beads (for GST-tagged proteins)

Baculovirus Protein Expression
• Clone gene of interest into an expression vector
• Generation, identification, and purification of recombinant clones
• Amplification for high titer stock
• Large-scale culture
• Purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione beads (for GST-tagged proteins)

Protein Expression in Mammalian Cells
• large scale cultivation of COS, CHO, or other mammalian cell lines
• Expression of your gene of intrest

Gene Expression in the Phoenix™ Retrovirus System
• Transfection of your gene of interest into the Phoenix™ Retrovirus System
• Optimization of your viral yield and protein expression

VALUE
Our goal is to help the proteomic community quickly and cost-effectively develop reagents to study novel gene targets. Our standard prices are among the lowest in the industry, but additional savings can be realized through our Collaboration Program.

INTEGRATION
BioCarta can be your single source for reagent development. Starting from a gene accession number, we can quickly and efficiently generate recombinant proteins in E. coli or baculovirus. The expressed proteins can then be utilized as antigens for polyclonal or monoclonal antibody production.

CONVENIENCE
Reduce administrative costs by consolidating your custom needs through BioCarta. No need to spend extra time and resources interfacing with multiple vendors. BioCarta will manage all projects for you, freeing up your time for additional research.