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BioCarta offers a menu of custom services designed to streamline the process of developing reagents to study new proteins, resulting from the explosion in the growth of new targets for academic study and drug development.




    Researchers require a large toolbox of well-characterized reagents to drive their functional proteomics research. To address this need, BioCarta provides over 18,000 products, including antibodies, proteins, cells, cell-based assays, and detection kits. BioCarta also offers a menu of custom services designed to streamline the process of developing reagents to study new proteins, resulting from the explosion in the growth of new targets for academic study and drug development.

    Polyclonal Antibody Development
    • Synthesis of up to 15aa peptide from customer supplied sequence
    • Includes conjugation of peptide to KLH via N-Terminal cysteine
    • 11 week, 2 rabbit protocol
    • ELISA test to determine specificity and titer
    • Protein G purification

    Monoclonal Antibody Development
    • Synthesis of up to 15aa peptide from customer supplied sequence
    • Includes conjugation of peptide to KLH via N-Terminal cysteine
    • Hybridoma fusion, screening, subcloning and expansion
    • Purification by Protein G

    *Please inquire for optional services, including affinity purification, extended immunization schedule, and synthesis of additional peptide.

    Bacteria Protein Expression
    • Clone gene of interest into an expression vector
    • Generation and identification of bacterial colonies expressing target proteins
    • Large-scale culture
    • Purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione beads (for GST-tagged proteins)

    Baculovirus Protein Expression
    • Clone gene of interest into an expression vector
    • Generation, identification, and purification of recombinant clones
    • Amplification for high titer stock
    • Large-scale culture
    • Purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione beads (for GST-tagged proteins)

    Protein Expression in Mammalian Cells
    • large scale cultivation of COS, CHO, or other mammalian cell lines
    • Expression of your gene of intrest

    Gene Expression in the Phoenix™ Retrovirus System
    • Transfection of your gene of interest into the Phoenix™ Retrovirus System
    • Optimization of your viral yield and protein expression

    Double Stranded RNA interference Oligo Synthesis
    • Fluorescent dsRNAi oligonucleotides against any gene of any organism

    Why Utilize BioCarta For Your Custom Service Projects?

    VALUE
    Our goal is to help the proteomic community quickly and cost-effectively develop reagents to study novel gene targets. Our standard prices are among the lowest in the industry, but additional savings can be realized through our Collaboration Program.

    INTEGRATION
    BioCarta can be your single source for reagent development. Starting from a gene accession number, we can quickly and efficiently generate recombinant proteins in E. coli or baculovirus. The expressed proteins can then be utilized as antigens for polyclonal or monoclonal antibody production.

    CONVENIENCE
    Reduce administrative costs by consolidating your custom needs through BioCarta. No need to spend extra time and resources interfacing with multiple vendors. BioCarta will manage all projects for you, freeing up your time for additional research.

    HIGH QUALITY
    All work is performed by proven and capable scientists with years of experience developing and managing projects for major suppliers of quality research tools.





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