:: 2'-5'oligoadenylate synthetase 2 (OAS2) polyclonal antibody

2'-5'oligoadenylate synthetase 2 (OAS2) polyclonal antibody

Animal ID:
RB1943/1944

Host:
Rabbit

Reactivity:
Human

Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.

Application:
Tested by peptide-specific ELISA (1:1,000). IHC (1:50 ~1:100)

Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 713 - 727 [NNSKRNFWRSSGNRF] of the human 2'-5'oligoadenylate synthetase 2 (OAS2) protein.

Accession number:
NM_002535

Description:
2'-5'-Oligoadenylate synthetases are interferon-induced enzymes that upon activation by double-stranded RNA polymerize ATP to 2'-5'-linked oligoadenylates. Three human OAS enzymes corresponding to proteins of 40/46, 69/71, and 100 kDa have been described. Based on the deduced amino acid sequences of the corresponding cDNAs, these OAS proteins share a homologous region of about 350 amino acid residues that represent the functional domain of OAS. The 40/46 kDa OAS proteins contain one single domain, whereas OAS2 belongs to the 69/71 kDa OAS proteins which contain two adjacent OAS domains. Some OAS proteins are 100-kDa proteins and contain three adjacent domains. OAS proteins play a significant role in the inhibition of cellular protein synthesis and viral infection resistance. Alternative splicing of the OAS2 gene produces two protein isoforms (p69 and p71) which share a common N-terminal sequence but diverge in the C-terminus. Only the p71 isoform is seen by this antibody.

Alternative Name(s):
P69/P71, 2'-5'-oligoadenylate synthetase 2 (69-71 kD), OAS2

References:

Hovnanian A, Rebouillat D, Mattei MG, Levy ER, Marie I, Monaco AP, Hovanessian AG: The human 2',5'-oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and 40-kDa forms. Genomics 52(3): 267-277 (1998).
Marie I, Hovanessian AG: The 69-kDa 2-5A synthetase is composed of two homologous and adjacent functional domains. J. Biol. Chem. 267(14): 9933-9939 (1992).
Marie I, Svab J, Robert N, Galabru J, Hovanessian AG: Differential expression and distinct structure of 69- and 100-kDa forms of 2-5A synthetase in human cells treated with interferon. J. Biol. Chem. 265(30): 18601-18607 (1990).
Marie I, Galabru J, Svab J, Hovanessian AG: Preparation and characterization of polyclonal antibodies specific for the 69 and 100 k-dalton forms of human 2-5A synthetase. Biochem. Biophys. Res. Commun. 160(2): 580-587 (1989).
Hovanessian AG, Svab J, Marie I, Robert N, Chamaret S, Laurent AG: Characterization of 69- and 100-kDa forms of 2-5A-synthetase from interferon-treated human cells. J. Biol. Chem. 63(10): 4959-4959 (1988).
Hovanessian AG, Laurent AG, Chebath J, Galabru J, Robert N, Svab J: Identification of 69-kd and 100-kd forms of 2-5A synthetase in interferon-treated human cells by specific monoclonal antibodies. EMBO J. 6(5): 1273-1280 (1987).

For Research Use Only. Not for Diagnostic or Therapeutic Use.