:: DNA cross-link repair 1C (DCLRE1C, PSO2, artemis) polyclonal antibody

Animal ID:
RC40104

Host:
Rabbit

Reactivity:
human

Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.

Application:
Tested by peptide-specific ELISA (1:1,000), WB (1:1,000).

Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 677 - 692 [GESIAVKKRKCSLLDT] of the human "DNA cross-link repair 1C (DCLRE1C, PSO2, artemis)" protein.

Accession number:
NM_022487

Description:
DNA cross-link repair 1C (DCLRE1C, PSO2, artemis) is involved in V(D)J recombination and DNA repair. DCLRE1C has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. Artemis mutations cause human T-B-severe combined immunodeficiency associated with increased cellular radiosensitivity (RS-SCID). DNA-PKcs regulates DCLRE1C by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in nonhomologous DNA end joining.

Alternative Name(s):
SCIDA, SNM1C, ARTEMIS, RS-SCID, DCLREC1C, FLJ11360, A-SCID, artemis protein

References:

Moshous D, Callebaut I, de Chasseval R, Corneo B, Cavazzana-Calvo M, Le Deist F, Tezcan I, Sanal O, Bertrand Y, Philippe N, Fischer A, de Villartay JP: Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency. Cell 105(2): 177-186 (2001).

For Research Use Only. Not for Diagnostic or Therapeutic Use.